AG Salditt

3D imaging of Heart and Brain by Phase Contrast X-ray Tomography

The HBRC houses a tomography (µ-CT) laboratory for 3D analysis of tissues and small animal. The laboratory is built up and operated by the group of Tim Salditt (Institute for X-ray Physics) and also part of the Multiscale Bioimaging Cluster of Excellence (MBExC). Beyond standard absorption contrast CT, the development and exploitation of advanced of phase contrast methods for histology and pathology in particular of the Brain and Heart is one of the goals persued within the MBExC .

Instruments:    

As a first μ-CT instrument the EasyTOM system (RX Solutions, France) is available for scans over a wide range of resolution and field of view, covering sub-micron scales to entire organ. The instrument is equipped with a microfocus (Hamamatsu L12161-07, W Target, 5 to 50 μm spotsize) and a nanofocus source (Hamamatsu L10711-02, W target, LaB6 cathode), as well as a CCD camera (4008 × 2672 pixels, 9 μm pixel size) equipped with a fiber coupled Gadox scintillator, or alternatively by a flat panel detector (1440 × 1704 pixels, 127 μm pixel size).

Fig.1 3D Imaging of guinea pig cochlea by x-ray phase contrast computed tomography for research targeting hearing loss. A volume rendering (NVIDIA IndeX) is shown for an overview scan recorded at 7.2µm voxel size with the microfocus source.   A noise induced pathology can be located then is investigated at higher resolution in subsequent scans. Based on staining (OTO stains), degeneration of neurons and pillar cells is visualized. This occurs in the sensory epithelium at the position corresponding to thefrequency peak of the traumatic noise spectrum.  Adpted from Schaeper et al, J.Medical Imaging 053501-2 (2023).

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AG-Leitung Tim Salditt

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